Difference between revisions of "Fastq mcf"
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Revision as of 17:17, 25 March 2019
Introduction
fastq-mcf attempts to:
* Detect & remove sequencing adapters and primers * Detect limited skewing at the ends of reads and clip * Detect poor quality at the ends of reads and clip * Detect Ns, and remove from ends * Remove reads with CASAVA 'Y' flag (purity filtering) * Discard sequences that are too short after all of the above * Keep multiple mate-reads in sync while doing all of the above