Difference between revisions of "Fastq mcf"

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(Created page with "=Introduction= fastq-mcf attempts to: * Detect & remove sequencing adapters and primers * Detect limited skewing at the ends of reads and clip * Detect poor quality at...")
 
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fastq-mcf attempts to:
 
fastq-mcf attempts to:
 
 
   * Detect & remove sequencing adapters and primers
 
   * Detect & remove sequencing adapters and primers
 
   * Detect limited skewing at the ends of reads and clip
 
   * Detect limited skewing at the ends of reads and clip

Revision as of 17:17, 25 March 2019

Introduction

fastq-mcf attempts to:

 * Detect & remove sequencing adapters and primers
 * Detect limited skewing at the ends of reads and clip
 * Detect poor quality at the ends of reads and clip
 * Detect Ns, and remove from ends
 * Remove reads with CASAVA 'Y' flag (purity filtering)
 * Discard sequences that are too short after all of the above
 * Keep multiple mate-reads in sync while doing all of the above
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